Cy5 and apc
WebPE-Cy5 (Ex-Max 496 nm/Em-Max 667 nm): is a tandem conjugate that combines phycoerythrin and a cyanine dye. Because of its broad absorption range and the fact that its emission spectrum is equivalent to APC, PE-Cy5 is not recommended for simultaneous … Web2 days ago · G At 5 weeks after the second immunization, 2 μg of PerCP-Cy5.5 conjugated CD45-specific antibody was injected i.v. via the tail vein 5 min before sacrifice. Lung cells and splenocytes were ...
Cy5 and apc
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WebBD Biosciences Pharmingen has maximized the fluorochrome energy transfer in PE-Cy5, thus maximizing its fluorescence emission intensity, minimizing residual emission from PE, and minimizing lot-to-lot variation. PE-Cy5 is optimized for use with a single argon ion … WebPeridinin chlorophyll protein-Cyanine5.5 (PerCP-Cyanine5.5) is a photostable tandem dye excited from the 488 nm laser. It is stable in fixative, and stained cells can be stored overnight. Less-abundant intracellular antigens stained with PerCP-Cyanine5.5 can often be reliably detected in multicolor flow cytometry experiments.
WebAPC (Allophycocyanin) is a fluorescent compound with an excitation peak at 651 nm and an emission peak at 660 nm. It can be excited using a 640 nm laser paired with a 670/30 nm bandpass filter, a configuration that can be found, for example, in the BD FACSAria™ Fusion. APC (Allophycocyanin) is spectrally similar to iFluor 633, Cy5 (Cyanine-5 ... WebApr 10, 2024 · 一.产品描述Cy5-PEG-DBCO，Cyanine5-PEG-DBCO，花青素Cy5-聚乙二醇-二苯并环辛炔，Cy5 (Cyanine 5) 是一种发远红(far-red)荧光的花青素荧光染料，分为普通Cy5和磺化Cy5(Sulfo-Cy5)。它的消光系数很高，荧光很亮，并且对pH不敏感，一般可 …
WebThe Cy5 HYQ filter combination is designed to optimize detection of fluorescence from the fluorochrome Cy5, and the set also provides excellent performance for the majority of fluorophores excited by red wavelengths. … WebThe bandpass of the new ''Cy5'' filter (A) is narrower (670/20 nm instead of 670/50 nm) so that a part of the APC emission is not measurable (Fig. 7). The resolution parameter of PE-Cy5 is...
WebIn instances where only the antibody exhibiting non-specific binding (for example, CD3 APC/Cy7, CD56 PE/Cy5 and CD19 PE/Dazzle™ 594) was used to costain cells with CD14 BV421™, the True-Stain Monocyte …
WebJan 1, 2009 · The FITC - APC pair is more commonly used in flow cytometry. FITC - Cy5 co-staining was used frequently in fluorescent microscopy however the advent of the Alexa-Fluor dyes is much more prevalent in fluoresent microscropy now. I would highly suggest using FITC and APC in your experiment. the cabin howell miWebJan 1, 2009 · The FITC - APC pair is more commonly used in flow cytometry. FITC - Cy5 co-staining was used frequently in fluorescent microscopy however the advent of the Alexa-Fluor dyes is much more prevalent in fluoresent microscropy now. I would highly suggest … tate of mind consultinghttp://www.protocol-online.org/biology-forums/posts/42100.html tate office chairWebThe first step of my whole project is to separate cells into different populations based on their markers so I will use multiple colours on them and it is hard to prevent overlaps. The other... tate officeWebOct 9, 2016 · A synthetic methodology of preparing novel membrane stable, responsive dyes is revealed in this manuscript. 1,3-bis (arylimino)isoindole dyes were synthesized and their properties to undergo... taten von martin luthertate office linebackerhttp://web.mit.edu/flowcytometry/www/Becton%20Dickinson%20Multicolor_AppNote.pdf tate oak and glass dining table