WebAug 30, 2024 · Founders Curmudgeon Better Half (Old Ale) clone recipe. Thread starter JMK1992; Start date Aug 14, 2024; Help Support Homebrew Talk - Beer, Wine, Mead, & Cider Brewing Discussion Forum: J. JMK1992 Active Member. Joined Jan 14, 2024 Messages 32 Reaction score 4. Aug 14, 2024 #1 WebMay 28, 2016 · Assemble the two overlapping fragments into a full-length cDNA clone using a unique restriction site that is incorporated into the PCR primers and another site that is naturally existing in the viral genome (Fig. 3). Set up the first digestion reaction using SacI for both the 5′ half clone and the 3′ half clone. Incubate the reaction at 37 ...
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