Pshuttle cmv gfp
WebDec 21, 2012 · pCMV-Sport6.1-Sdc1 was purchased from ATCC (catalog number 10326966), and the Sdc1 gene was isolated by KpnI and XhoI and reconstructed into the shuttle plasmid, pXhuttle-CMV-GFP (SinoGenoMax Co, Ltd) to form the product, pShuttle-CMV-GFP-Sdc1. CMV-GFP-Sdc1 was isolated from this product and ligated into the ademovirus vector, … WebA therapeutic gene and tissue-specific promoter (TSP) could be inserted routinely into the MCS of pShuttle-CMV and pTE-ME1, respectively. The modified E1 region could then be …
Pshuttle cmv gfp
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WebJan 29, 2008 · For both HeLa and 911HER cells transfection with pShuttle-CMV5-GFP resulted in greater production of eGFP as shown in Fig. 1. However, the relative difference between eGFP expression by pAdTrack (CMV-eGFP) and pShuttle-CMV5-GFP in HeLa and in 911HER cells indicates that CMV5 and CMV promoters behave differently in those two … WebpAdEasy & pShuttle Vectors RUO Recombinant adenoviruses are a versatile tool for gene delivery and expression. Choose pAdEasy-1 vector or any of these three shuttle vectors, …
WebPlasmid pShuttle-CMV-EGFP-C from Dr. Lorene Lanier's lab contains the insert C domain of N-WASP and is published in Neuron. 2004 Jul 8. 43(1):81-94. This plasmid is available … WebPshuttle Cmv Egfp Shuttle Vector, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, …
WebApr 12, 2016 · The plasmid expressing glutathione S-transferase (GST)-tagged human nATF6 (GST-nATF6) was constructed by cloning the human ATF6 cDNA encoding amino acids 1 to 373 into the Cla I/ Not I sites of pEBG (a GST fusion vector). The plasmids encoding pSG5-PPARα ( 22) and Sport-RXRα ( 22) were purchased from Addgene … WebJul 28, 2024 · Mixed populations of cells were transfected with rAd-GFP (100 PFU/cell), as a negative control - cells without transduction (no Ad). After 24 h, a mean fluorescence intensity of GFP ( b, c) and percentage of GFP-positive cells ( d) were measured in both populations of cell.
WebAfter identification by Sfi I digestion and sequencing, pShuttle-TFRC-Luciferase vector and the adenoviral backbone vector (pAdeno) were subjected to homologous recombination. The correct recombinant plasmid was then transfected into 293 packaging cells to produce adenoviral particles and confirmed by PCR.
WebpShuttle-CMV-lacZ control vector (Catalog #240008; 1 μg/μl in TE buffer) 10 μg BJ5183 electroporation competent cellsa (Catalog #200154) 5 × 100 μl (Clear tubes) XL10-Gold … morrowind iso download pcWebany of the four shuttle vectors (pShuttle-CMV, pShuttle, pShuttle-IRES-hrGFP-1 (Catalog #240081) or pShuttle-IRES-hrGFP-2 (Catalog #240082). Once constructed, the shuttle … morrowind item idWebAug 1, 2006 · To generate pAdenoVator-CMV5-V-EGFP, pShuttle-E1-CMV-V-EGFP was cut with SalI/blunt/BstXI to give a fragment with the fusion gene and then ligated into pAdenoVator cut with PmeI/BstXI. Finally, a parent E3 shuttle plasmid was made to insert the same fusion genes into the adenovirus E3 region by double selection homologous … morrowind item enchantment point listWeb同时pLenti-H1含有CMV启动子驱动表达的绿色荧光蛋白基因GFP。 直接转染哺乳动物细胞后或者包装好的病毒感染哺乳动物细胞后，CMV启动子能高效启动绿色荧光蛋白GFP的表 … minecraft pocket edition download pc javaWebJun 14, 2004 · Recombinant Replication-Deficient Adenoviruses Expressing Syncollin–GFP. Syncollin–pEGFP-N1 was transformed into DM1 cells and subsequently subcloned into pShuttle–CMV adenoviral vector (Quantum Biotechnologies, Montreal) . Recombinant adenovirus was generated by using the bacterial recombination method as described . morrowind item listWebJan 7, 2024 · Product Name : pShuttle article : bacterial resistance : Kanamycin cloning : growth notes : Vector pShuttle is used for expression of transgenes under a chosen (non … morrowind items console commandiWebpBADMycHisA pQE2 pQE9 pQE30lacIq pQE30 pQE31 pQE32 pQE40 pQE60 pQE70 pQE80L pQETrisystem pEZZ18 pSE380 pWHM3 pQBI63 pTrc99A pTrcMECT pTrc pTYB1 pTYB2 pTYB11 pTYB12 pCYB1 pBAD18 pBAD24 pBAD33 pBAD43 pBAD24-GFP pDest15-N-throbin pDest22 pDest32 pED-Trx-pp-air pED-pp morrowind ita download