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Putative endolysin

WebApr 12, 2011 · We found that an endolysin gene (psm) in phiSM101, an episomal phage of enterotoxigenic C. perfringens type A strain, SM101 (Myers et al. 2006), encodes a … WebThe 723 putative endolysins ranged from 72 to 578 residues in length, using 24 different catalytic domains and 18 different cell wall-binding domains organized with 89 different …

A Putative Amidase Endolysin Encoded by Clostridium ... - PubMed

WebMar 15, 2024 · Bioinformatics analysis using BLAST with LysK endolysin gene sequence as query allowed for the identification of 25 putative endolysin encoding genes. Cg and … is email home safe https://gtosoup.com

Phage lysin that specifically eliminates Clostridium botulinum …

WebThis study investigates 2,628 putative endolysin genes identified in 183,298 bacteriophage genomes. Previously, bioinformatic approaches resulted in a database of 66 SAR-endolysins. WebAug 20, 2024 · Clostridioides difficile is the major pathogen of pseudomembranous colitis, and novel antimicrobial agents are sought after for its treatment. Phage-derived … WebMay 7, 2024 · Dataset. We evaluated 2628 putative endolysin genes found in 183,298 genomic sequences of uncultured viral genomes previously identified by Fernández-Ruiz … is email in the dictionary

A Thermostable Salmonella Phage Endolysin, Lys68, with Broad

Category:The Formulation of Bacteriophage in a Semi Solid Preparation for …

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Putative endolysin

A Putative Amidase Endolysin Encoded by Clostridium perfringens …

WebA Putative Amidase Endolysin Encoded by Clostridium perfringens St13 Exhibits Specific Lytic Activity and Synergizes with the Muramidase Endolysin Psm Hiroshi Sekiya et al. Antibiotics, 2024 Ts2631 Endolysin from the Extremophilic Thermus scotoductus Bacteriophage vB_Tsc2631 as an Antimicrobial Agent against Gram-Negative Multidrug … WebOct 7, 2014 · Resistance rates are increasing among several problematic Gram-negative pathogens, a fact that has encouraged the development of new antimicrobial agents. This paper characterizes a Salmonella phage endolysin (Lys68) and demonstrates its potential antimicrobial effectiveness when combined with organic acids towards Gram-negative …

Putative endolysin

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WebMar 1, 2024 · Clostridium perfringens is an often-harmful intestinal bacterium that causes various diseases ranging from food poisoning to life-threatening fulminant disease. … WebMar 22, 2012 · Computational predictions and analyses of HolSMP. The complete 36,126-bp sequence of phage SMP has 48 open reading frames (ORF). ORF42, designated LySMP, encodes the putative endolysin.ORF43, which is 429 bp long and upstream of the lySMP gene, is predicted to encode a putative holin protein, designated HolSMP (Figure …

WebDec 9, 2024 · Here we introduce a putative phage lysin that specifically lyses vegetative C. botulinum Group I cells. This lysin, called CBO1751, efficiently kills cells of C. botulinum Group I strains at the ... WebApr 11, 2024 · Aiming at utilizing a phage endolysin as a therapeutic alternative to antibiotics, we surveyed the genome and bacteriophage sequences of C. perfringens. A phiSM101 muramidase gene (psm) revealed by this study can be assumed to encode an N-acetylmuramidase, since the N-terminal catalytic domain deduced from the gene shows …

WebMar 1, 2024 · that a 36.1-kbp putative phage remnant region exists only on the genome of C. perfringens st13 and is absent from the genomes of the other sequenced C. perfringens strains (Figure S2). A gene designated psa (gene ID; CPE1138), encoding a putative endolysin, was detected at one end of this region; a putative holin gene is also present in … WebApr 10, 2024 · As a result, any dynamic endolysin-based infection power merchants must combine outer-membrane-permeabilizing marketers after remaining ... Wang G et al (2024) Biological properties and genomics analysis of vB_KpnS_GH-K3, a Klebsiella phage with a putative depolymerase-like protein. Virus Genes 55:696–706. Article CAS PubMed ...

WebMay 25, 2024 · Bacteriophage encoded endolysin as an alternative antibiotics is a peptidoglycan degrading enzyme with a low rate of resistance. Here, the engineered endolysin was developed to defeat multiple drug-resistant (MDR) Acinetobacter baumannii. First, putative endolysin PA90 was predicted by genome analysis of isolated …

WebDec 9, 2024 · Here we introduce a putative phage lysin that specifically lyses vegetative C. botulinum Group I cells. This lysin, called CBO1751, efficiently kills cells of C. botulinum … ryan wilson cbssportsWebClostridium perfringens is an often-harmful intestinal bacterium that causes various diseases ranging from food poisoning to life-threatening fulminant disease. Potential … ryan wilson dc oagWebAug 1, 2011 · The putative endolysin gene (ORF24) previously determined in the phage BFK20 genome (EMBL accession no. AJ278322) had to be corrected from 576 bp (ORF24) to 813 bp (ORF24′) because sequencing errors were detected which resulted in a frameshift mutation. The endolysin of BFK20 (gp24′) contains 270 aa, which corresponds to a 30.1 … ryan wilson elo chessWebMar 1, 2024 · A Putative Amidase Endolysin Encoded by Clostridium perfringens St13 Exhibits Specific Lytic Activity and Synergizes with the Muramidase Endolysin Psm ... is email on acid a crtmWebPreviously, bioinformatic approaches resulted in a database of 66 SAR endolysins. This manuscript almost doubles the list with 53 additional SAR endolysin candidates. Forty … is email on deck safeWebSep 1, 2024 · Identification and overexpression of recombinant endolysin LysSTG2. From the genome sequence of phage STG2, the predicted open reading frame for a putative endolysin gene encoding a protein (LysSTG2) consisting of 138 amino acids (expected molecular mass of 15.3 kDa) was identified. is email considered private informationWebFeb 15, 2024 · The gene (GenBank accession no. OL415943) encoding putative endolysin from bacteriophage PBEC131 was cloned into pET21a+ (Novagen, United States) using … ryan wilson etsu