WebJul 14, 2015 · A total of 14 regenerated rice plants were obtained after Agrobacterium-mediated transformation and hygromycin selection. Gene integration and excision from T-DNA-regenerated rice plants was first verified by PCR with genomic DNA extraction from fresh leaves using P35SF and TCR primers specific for the CaMV 35S promoter and NtTC … WebDec 1, 2024 · Context. Rice ratooning produces a ratoon crop (RC) with regenerated tillers in different nodes on the stubble of main crop (MC). It is crucial to apply nitrogen (N) at 15 d after heading and 1–2 d after harvest of MC for promoting bud initiation (N B) and tiller growth (N T), respectively to achieve high grain yield of RC.
Selected rice callus from the third selection cycle.
WebDec 30, 2011 · Rice transformation using Agrobacterium tumefaciens is a method of choice due to stable and low copy number integration of transfer-DNA (T-DNA) into the plant … WebThe results reveal that the resistance in regenerated rice plantlets to P. oryzae pathogen segregated as 1 resistant: 2 moderate resistant: 1 susceptible to P. oryzae may be controlled by one pair ... cooler waters movie
Genomic Variations of Rice Regenerants from Tissue Culture …
WebJul 21, 2024 · Although progenies from micropropagation are supposed to be identical at the genetic level, i.e., genetic clones, variations frequently occur. For example, rice … WebApr 1, 2014 · Background Tos17 was the first LTR retrotransposon (Copia) described as active in cultivated rice, and is present in two copies in the genome of the sequenced Nipponbare variety. Only the chromosome 7 copy is active and able to retrotranspose, at least during in vitro culture, and this ability was widely used in insertional mutagenesis … WebApr 14, 2024 · Similar to the result of genome analysis of regenerated rice (Miyao et al. 2012) or Arabidopsis (Zhang et al. 2014), some variations were inherited in progenies of the regenerated Chinese cabbage plants. Table 4 Description of the exonic SNP candidates identified in the regenerated R 0 plants and their progenies. cooler water pump price amazon