Web25. apr 2013 · TopHat is a popular spliced aligner for RNA-sequence (RNA-seq) experiments. In this paper, we describe TopHat2, which incorporates many significant enhancements to TopHat. TopHat2 can align reads of various lengths produced by the latest sequencing technologies, while allowing for variable-length indels with respect to the reference genome. http://www.360doc.com/content/18/0714/20/19913717_770401548.shtml
Tophat2比对原理及命令
WebTopHat needs you specify a path to the index files and an input file containing your reads. The first argument should be the full path to the directory containing the index plus the prefix of the index files. To start the TopHat pipeline, enter the command: tophat /path/to/h_sapiens reads1.fq,reads2.fq,reads3.fq. http://blog.sina.com.cn/s/blog_6836e9f40102v4hb.html mouldking facebook
lncRNA 全基因组鉴定番茄果实lncRNA,及与调节果实成熟相 …
Web3. nov 2015 · Apache是有C语言实现的,支持各种特性和模块从而来扩展核心功能;Tomcat是Java编写的,更好的支持Servlet和JSP。. 1、Apache是Web服务器,Web服务器传送 (serves)页面使浏览器可以浏览,Web服务器专门处理HTTP请求 (request),但是应用程序服务器是通过很多协议来为应用 ... Web3、tophat2比对 #比对NAT sample #-G gtf文件;-o bowtie建立参考基因组索引的公共名(前缀); nohup tophat2 -p 6 -G /data2/xxx/data2/mm10/gtf/mm10_genes.gtf / -o ./NAT /data2/xxx/data2/mm10/mm10_index/mm10_genome_bowtie2 / /data2/xxx/data2/2cutadapt/NAT-RNA_combined_clean_R1.fastq / … Tophat 首次被发表已经是6年前 Cufflinks也是五年前的事情了 Star的比对速度是tophat的50倍,hisat更是star的1.2倍。 stringTie的组装速度是cufflinks的25倍,但是内存消耗却不到其一半。 Ballgown在差异分析方面比cuffdiff更高的特异性及准确性,且时间消耗不到cuffdiff的千分之一 Bowtie2+eXpress做质量 … Zobraziť viac mould king eclipse